Galactooligosaccharides formation during enzymatic hydrolysis of lactose: Towards a prebiotic-enriched milk

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Galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic-enriched milk.

The formation of galactooligosaccharides (GOS) in skim milk during treatment with several commercial β-galactosidases (Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae) was analysed in detail, at 4 and 40°C. The maximum GOS concentration was obtained at a lactose conversion of approximately 40-50% with B. circulans and A. oryzae β-galactosidases, and at 95% lactose depletion for ...

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Galactosyl-oligosaccharide formation during lactose hydrolysis: a review

Enzymatic hydrolysis of lactose is accompanied by galactosyl transfer to other sugars, thereby producing oligosaccharides. These are hydrolyzed slowly, both in vitro and in vivo. They can be thought of as low molecular weight, non-viscous, water-soluble, dietary fibre. They are considered to be physiologically functional foods which promote the growth of bifidobacteria in the colon and a wide v...

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Effect of lactose hydrolysis on calcium absorption during duodenal milk perfusion.

A multi-lumen intubation system was used to study the absorption of calcium, glucose and galactose in 13 human subjects. The intubation was placed between the duodenum abdomen and proximal jejunum and the subjects were perfused with milk and lactase-supplemented milk. Lactose disappearance over a 20 cm length of intestine was used as the index of lactase activity. The subjects were assigned to ...

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Standardization of different levels of lactose hydrolysis in the preparation of lactose hydrolyzed yoghurt

Yoghurt is a unique fermented dairy product, consumed throughout the world and the trend ofconsumption is increasing due to its unique health benefits. Yoghurt mix is often supplemented with MSNF(milk solids-non-fat), it may contain as much as 5.7% unhydrolysed lactose. Lactose can be hydrolysed withan enzyme lactase. Deficiency of lactase in lactose-intolerant persons leads to gastrointestinal...

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A new kinetic model proposed for enzymatic hydrolysis of lactose by a -galactosidase from Kluyveromyces fragilis

We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25–40 ◦C), enzyme concentration (0.1–3.0 g l−1), lactose concentration (0.0278–0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis–Me...

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ژورنال

عنوان ژورنال: Food Chemistry

سال: 2014

ISSN: 0308-8146

DOI: 10.1016/j.foodchem.2013.08.060